Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.
Identifieur interne : 001A24 ( Main/Exploration ); précédent : 001A23; suivant : 001A25Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.
Auteurs : Michael Thorsen [Suède] ; Therese Jacobson ; Riet Vooijs ; Clara Navarrete ; Tijs Bliek ; Henk Schat ; Markus J. TamásSource :
- Molecular microbiology [ 1365-2958 ] ; 2012.
Descripteurs français
- KwdFr :
- Antifongiques (antagonistes et inhibiteurs), Antifongiques (métabolisme), Arsénites (antagonistes et inhibiteurs), Arsénites (métabolisme), Glutathion (métabolisme), Inactivation métabolique (MeSH), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Saccharomyces cerevisiae (métabolisme).
- MESH :
- antagonistes et inhibiteurs : Antifongiques, Arsénites.
- croissance et développement : Saccharomyces cerevisiae.
- effets des médicaments et des substances chimiques : Saccharomyces cerevisiae.
- métabolisme : Antifongiques, Arsénites, Glutathion, Saccharomyces cerevisiae.
- Inactivation métabolique.
English descriptors
- KwdEn :
- Antifungal Agents (antagonists & inhibitors), Antifungal Agents (metabolism), Arsenites (antagonists & inhibitors), Arsenites (metabolism), Glutathione (metabolism), Inactivation, Metabolic (MeSH), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Antifungal Agents, Arsenites.
- chemical , metabolism : Antifungal Agents, Arsenites, Glutathione.
- drug effects : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Inactivation, Metabolic.
Abstract
Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid is ubiquitous in nature, virtually all living organisms require systems for detoxification and tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports and accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation of the arsenite triglutathione complex As(GS)₃ was also detected and direct transport assays demonstrate that As(GS)₃ does not readily enter cells. Yeast cells with increased extracellular GSH levels accumulate less arsenic and display improved growth when challenged with As(III). Conversely, cells defective in export and extracellular accumulation of GSH are As(III) sensitive. Taken together, our data are consistent with a novel detoxification mechanism in which GSH is exported to protect yeast cells from arsenite toxicity by preventing its uptake.
DOI: 10.1111/j.1365-2958.2012.08085.x
PubMed: 22554109
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.</title><author><name sortKey="Thorsen, Michael" sort="Thorsen, Michael" uniqKey="Thorsen M" first="Michael" last="Thorsen">Michael Thorsen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Molecular Biology, University of Gothenburg, S-40530 Gothenburg, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry and Molecular Biology, University of Gothenburg, S-40530 Gothenburg</wicri:regionArea><wicri:noRegion>S-40530 Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Jacobson, Therese" sort="Jacobson, Therese" uniqKey="Jacobson T" first="Therese" last="Jacobson">Therese Jacobson</name></author><author><name sortKey="Vooijs, Riet" sort="Vooijs, Riet" uniqKey="Vooijs R" first="Riet" last="Vooijs">Riet Vooijs</name></author><author><name sortKey="Navarrete, Clara" sort="Navarrete, Clara" uniqKey="Navarrete C" first="Clara" last="Navarrete">Clara Navarrete</name></author><author><name sortKey="Bliek, Tijs" sort="Bliek, Tijs" uniqKey="Bliek T" first="Tijs" last="Bliek">Tijs Bliek</name></author><author><name sortKey="Schat, Henk" sort="Schat, Henk" uniqKey="Schat H" first="Henk" last="Schat">Henk Schat</name></author><author><name sortKey="Tamas, Markus J" sort="Tamas, Markus J" uniqKey="Tamas M" first="Markus J" last="Tamás">Markus J. Tamás</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22554109</idno><idno type="pmid">22554109</idno><idno type="doi">10.1111/j.1365-2958.2012.08085.x</idno><idno type="wicri:Area/Main/Corpus">001992</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001992</idno><idno type="wicri:Area/Main/Curation">001992</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001992</idno><idno type="wicri:Area/Main/Exploration">001992</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.</title><author><name sortKey="Thorsen, Michael" sort="Thorsen, Michael" uniqKey="Thorsen M" first="Michael" last="Thorsen">Michael Thorsen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Molecular Biology, University of Gothenburg, S-40530 Gothenburg, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry and Molecular Biology, University of Gothenburg, S-40530 Gothenburg</wicri:regionArea><wicri:noRegion>S-40530 Gothenburg</wicri:noRegion></affiliation></author><author><name sortKey="Jacobson, Therese" sort="Jacobson, Therese" uniqKey="Jacobson T" first="Therese" last="Jacobson">Therese Jacobson</name></author><author><name sortKey="Vooijs, Riet" sort="Vooijs, Riet" uniqKey="Vooijs R" first="Riet" last="Vooijs">Riet Vooijs</name></author><author><name sortKey="Navarrete, Clara" sort="Navarrete, Clara" uniqKey="Navarrete C" first="Clara" last="Navarrete">Clara Navarrete</name></author><author><name sortKey="Bliek, Tijs" sort="Bliek, Tijs" uniqKey="Bliek T" first="Tijs" last="Bliek">Tijs Bliek</name></author><author><name sortKey="Schat, Henk" sort="Schat, Henk" uniqKey="Schat H" first="Henk" last="Schat">Henk Schat</name></author><author><name sortKey="Tamas, Markus J" sort="Tamas, Markus J" uniqKey="Tamas M" first="Markus J" last="Tamás">Markus J. Tamás</name></author></analytic><series><title level="j">Molecular microbiology</title><idno type="eISSN">1365-2958</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (antagonists & inhibitors)</term><term>Antifungal Agents (metabolism)</term><term>Arsenites (antagonists & inhibitors)</term><term>Arsenites (metabolism)</term><term>Glutathione (metabolism)</term><term>Inactivation, Metabolic (MeSH)</term><term>Saccharomyces cerevisiae (drug effects)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Saccharomyces cerevisiae (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (antagonistes et inhibiteurs)</term><term>Antifongiques (métabolisme)</term><term>Arsénites (antagonistes et inhibiteurs)</term><term>Arsénites (métabolisme)</term><term>Glutathion (métabolisme)</term><term>Inactivation métabolique (MeSH)</term><term>Saccharomyces cerevisiae (croissance et développement)</term><term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term><term>Saccharomyces cerevisiae (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Antifungal Agents</term><term>Arsenites</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antifungal Agents</term><term>Arsenites</term><term>Glutathione</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Antifongiques</term><term>Arsénites</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antifongiques</term><term>Arsénites</term><term>Glutathion</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Inactivation, Metabolic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Inactivation métabolique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid is ubiquitous in nature, virtually all living organisms require systems for detoxification and tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports and accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation of the arsenite triglutathione complex As(GS)₃ was also detected and direct transport assays demonstrate that As(GS)₃ does not readily enter cells. Yeast cells with increased extracellular GSH levels accumulate less arsenic and display improved growth when challenged with As(III). Conversely, cells defective in export and extracellular accumulation of GSH are As(III) sensitive. Taken together, our data are consistent with a novel detoxification mechanism in which GSH is exported to protect yeast cells from arsenite toxicity by preventing its uptake.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22554109</PMID><DateCompleted><Year>2012</Year><Month>09</Month><Day>28</Day></DateCompleted><DateRevised><Year>2014</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2958</ISSN><JournalIssue CitedMedium="Internet"><Volume>84</Volume><Issue>6</Issue><PubDate><Year>2012</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Molecular microbiology</Title><ISOAbbreviation>Mol Microbiol</ISOAbbreviation></Journal><ArticleTitle>Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.</ArticleTitle><Pagination><MedlinePgn>1177-88</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-2958.2012.08085.x</ELocationID><Abstract><AbstractText>Arsenic is an environmental toxin and a worldwide health hazard. Since this metalloid is ubiquitous in nature, virtually all living organisms require systems for detoxification and tolerance acquisition. Here, we show that during chronic exposure to arsenite [As(III)], Saccharomyces cerevisiae (budding yeast) exports and accumulates the low-molecular-weight thiol molecule glutathione (GSH) outside of cells. Extracellular accumulation of the arsenite triglutathione complex As(GS)₃ was also detected and direct transport assays demonstrate that As(GS)₃ does not readily enter cells. Yeast cells with increased extracellular GSH levels accumulate less arsenic and display improved growth when challenged with As(III). Conversely, cells defective in export and extracellular accumulation of GSH are As(III) sensitive. Taken together, our data are consistent with a novel detoxification mechanism in which GSH is exported to protect yeast cells from arsenite toxicity by preventing its uptake.</AbstractText><CopyrightInformation>© 2012 Blackwell Publishing Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thorsen</LastName><ForeName>Michael</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Molecular Biology, University of Gothenburg, S-40530 Gothenburg, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jacobson</LastName><ForeName>Therese</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Vooijs</LastName><ForeName>Riet</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Navarrete</LastName><ForeName>Clara</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Bliek</LastName><ForeName>Tijs</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Schat</LastName><ForeName>Henk</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Tamás</LastName><ForeName>Markus J</ForeName><Initials>MJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>05</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Microbiol</MedlineTA><NlmUniqueID>8712028</NlmUniqueID><ISSNLinking>0950-382X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018053">Arsenites</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical><Chemical><RegistryNumber>N5509X556J</RegistryNumber><NameOfSubstance UI="C015001">arsenite</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018053" MajorTopicYN="N">Arsenites</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008658" MajorTopicYN="N">Inactivation, Metabolic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>5</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>9</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22554109</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2958.2012.08085.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Bliek, Tijs" sort="Bliek, Tijs" uniqKey="Bliek T" first="Tijs" last="Bliek">Tijs Bliek</name><name sortKey="Jacobson, Therese" sort="Jacobson, Therese" uniqKey="Jacobson T" first="Therese" last="Jacobson">Therese Jacobson</name><name sortKey="Navarrete, Clara" sort="Navarrete, Clara" uniqKey="Navarrete C" first="Clara" last="Navarrete">Clara Navarrete</name><name sortKey="Schat, Henk" sort="Schat, Henk" uniqKey="Schat H" first="Henk" last="Schat">Henk Schat</name><name sortKey="Tamas, Markus J" sort="Tamas, Markus J" uniqKey="Tamas M" first="Markus J" last="Tamás">Markus J. Tamás</name><name sortKey="Vooijs, Riet" sort="Vooijs, Riet" uniqKey="Vooijs R" first="Riet" last="Vooijs">Riet Vooijs</name></noCountry><country name="Suède"><noRegion><name sortKey="Thorsen, Michael" sort="Thorsen, Michael" uniqKey="Thorsen M" first="Michael" last="Thorsen">Michael Thorsen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/DetoxFungiV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A24 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001A24 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= DetoxFungiV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22554109
|texte= Glutathione serves an extracellular defence function to decrease arsenite accumulation and toxicity in yeast.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22554109" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a DetoxFungiV1
| This area was generated with Dilib version V0.6.38. |  |